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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 保存条件:
Store at -80°C.
- 克隆性:
Recombinant
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 宿主:
Rabbit
- 应用范围:
WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
- 靶点:
SCF
- 抗体英文名:
Rat SCF Recombinant monoclonal antibody, PBS Only (Detector)
- 抗体名:
Rat SCF Recombinant monoclonal antibody, PBS Only (Detector)
- 规格:
100ug
产品信息
83707-2-PBS targets SCF as part of a matched antibody pair:
MP00664-1: 83707-1-PBS capture and 83707-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | KIT ligand |
| 别名 | Kitlg, Mgf, Kitl, KIT ligand, 240787H1 |
| 计算分子量 | 31kDa |
| 观测分子量 | 28 kDa, 45 kDa |
| GenBank蛋白编号 | NM_021843.4 |
| 基因名称 | Kitlg |
| Gene ID (NCBI) | 60427 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P21581-1 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis. SCF is a 35 to 45 kDa monomeric glycoprotein.
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文献和实验)、干细胞生长因子(SCF)、血小板生成素 (TPO)等。 5、趋化因子(chemokine) 主要功能是招募血液中的单核细胞、中性粒细胞、淋巴细胞等进入特定的淋巴器官和组织以及感染发生的部位。根据趋化因子近N端半胱氨酸(Cys)的位置、排列方式和数量,可分为CC、CXC、C、CX3C四个亚家族。 6生长因子(growth factor,GF) 生长因子(GF)是具有刺激细胞生长作用的细胞因子。 五、细胞因子的生物学活性 1.介导自然免疫、参与抗肿瘤和抗感染 2.调节T、B
, 0.1mM ßME. plus 3 U/mL Epo, 50 ng/mL Tpo, 100 ng/mL SCF, 100 ng/mL IL-6, ? ng/mL IL-3. Antibodies for Lineage Depletion: (Pharmingen) Antibodies for Sorting: (Pharmingen monoclonal rat anti-mouse)
为最大程度的保证实验结果,我们推荐客户严格遵循 CST 的实验操作方法,这些实验方法经过 CST 科学家的反复验证,能够保证实验结果的精确性和可重复性。 流式细胞术方案 A. 所需溶液和试剂 1. 1X 磷酸盐缓冲液(PBS):将 8 g 氯化钠(NaCl),0.2 g 氯化钾(KCl),1.44 g 磷酸氢二钠(Na2 HPO4),0.24 g 磷酸二氢钾(KH2PO4)溶解在 800 ml 蒸馏水(dH2O)中。用盐酸调节 pH 到 7.4,定容
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