- ￥920 - 2310
- Proteintech
- CR-65075
- 2025年10月09日
- FC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Cardinal Red™ Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
ICAM-1/CD54
- 抗体英文名：
Cardinal Red™ Anti-Human ICAM-1/CD54 (15.2)
- 抗体名：
Cardinal Red™ Anti-Human ICAM-1/CD54 (15.2)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥920.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥2310.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CR-65075 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | intercellular adhesion molecule 1 |
| 别名 | CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1 |
| 计算分子量 | 90 kDa |
| GenBank蛋白编号 | BC015969 |
| 基因名称 | ICAM-1 |
| Gene ID (NCBI) | 3383 |
| ENSEMBL Gene ID | ENSG00000090339 |
| RRID | AB_2935221 |
| 偶联类型 | Cardinal Red™ Fluorescent Dye |
| 最大激发/发射波长 | 592 nm / 611 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P05362 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).
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文献和实验off the solution, wash 2 times 5 min. each in 1x PBS, followed by 1 wash in 100mM Tris pH 8.2 for 5 min. Make up Vector Red substrate solution (Catalog No. SK-5100) immediately before use. Incubate in the dark for 20-30 min, stop color reaction by blotting
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
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