- ￥5800
- Proteintech
- 60774-2-PBS
- 2025年10月09日
- Cytometric bead array, Indirect ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SMARCA2-Specific protein
- 亚型：
IgG1
- 保存条件：
Store at -80°C.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
Cytometric bead array, Indirect ELISA
- 靶点：
SMARCA2-Specific
- 抗体英文名：
SMARCA2-Specific Monoclonal antibody, PBS Only (Detector)
- 抗体名：
SMARCA2-Specific Monoclonal antibody, PBS Only (Detector)
- 规格：
100ug
产品信息
60774-2-PBS targets SMARCA2-Specific as part of a matched antibody pair:
MP51103-1: 60774-1-PBS capture and 60774-2-PBS detection (validated in Cytometric bead array)
MP51103-2: 60774-3-PBS capture and 60774-2-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag33074 Product name: Recombinant human SMARCA2-Specific protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-272 aa of NM_003070 Sequence: MSTPTDPGAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPMPTMGSTDFPQEGMHQMHKPIDGIHDKGIVEDIHCGSMKGTGMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPMSGGGPTPPQMPPSQPGALIPGDPQAMSQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGP |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 |
| 别名 | SMARCA2, BRM, BRG1-associated factor 190B, BAF190B, BAF190 |
| 计算分子量 | 181 kDa |
| GenBank蛋白编号 | NM_003070 |
| 基因名称 | SMARCA2 |
| Gene ID (NCBI) | 6595 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G Magarose purification |
| UNIPROT ID | P51531 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
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文献和实验1. 杂交瘤阳性克隆的筛选与克隆化 在HAT培养基中生长的杂交瘤细胞，只有少数是分泌预定特异性单克隆抗体的细胞，因此，必须进行筛选和克隆化。通常采用有限稀释法进行杂交瘤细胞的克隆化培养。采用灵敏、快速、特异的免疫学方法，筛选出能产生所需单克隆抗体的阳性杂交瘤细胞，并进行克隆扩增。经过全面鉴定其所分泌单克隆抗体的免疫球蛋白类型、亚类、特异性、亲和力、识别抗原的表位及其分子量后，及时进行冻存。2. 单克隆抗体的大量制备 单克隆抗体的大量制备重要采用动物体内诱生法和体外培养法。
过夜。（5）用少许PBS将交联物全部洗出，收集洗出液，加1/20V体积新鲜配制的5mg/ml NaBH4溶液，混匀，室温作用30分钟；再加入3/20V NaBH4溶液，混匀，室温作用1小时（或4℃过夜）。（6）将交联物过Sephadex G200或Sepharose 6B（2.6×50cm）层析纯化，分管收集第一峰。（7）酶结合物质量鉴定：克分子比值测定酶量（mg/ml）=OD403×0.4IgG量（mg/ml）=（OD280-OD403×0.3）×0.62克分子比值（E/P）=酶量×4/IgG量
Blocking of unwanted non-specific staining
is not. Blocking of endogenous peroxidase is done by preincubating the slides in 3 H2O2 1 Sodium Azide PBS 30 minutes several washes This mixture is more effective than 1.5 H2O2 in absolute methanol Ref. Methanol incubation is also a fixation step
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