- ￥5800
- Proteintech
- 85738-1-PBS
- 2025年10月09日
- WB, Cytometric bead array, Indirect ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG
- 保存条件：
Store at -80°C.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 宿主：
Rabbit
- 应用范围：
WB, Cytometric bead array, Indirect ELISA
- 靶点：
CXCL4/PF4
- 抗体英文名：
Mouse CXCL4/PF4 Recombinant monoclonal antibody, PBS Only (Capture)
- 抗体名：
Mouse CXCL4/PF4 Recombinant monoclonal antibody, PBS Only (Capture)
- 规格：
100ug
产品信息
85738-1-PBS targets CXCL4/PF4 as part of a matched antibody pair:
MP02069-1: 85738-1-PBS capture and 85738-2-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Cytometric bead array, Indirect ELISA |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Eg3345 Product name: Recombinant Mouse CXCL4/PF4 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 30-105 aa of NM_019932 Sequence: VTSAGPEESDGDLSCVCVKTISSGIHLKHITSLEVIKAGRHCAVPQLIATLKNGRKICLDRQAPLYKKVIKKILES |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | platelet factor 4 |
| 别名 | C-X-C motif chemokine 4, CXCL4, PF-4, Platelet factor 4 |
| 计算分子量 | 11kd |
| 观测分子量 | 10-14 kDa |
| GenBank蛋白编号 | NM_019932 |
| 基因名称 | Pf4 |
| Gene ID (NCBI) | 56744 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Z126 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
The chemokine (C-X-C motif) ligand 4 (CXCL4) is also named as PF4 (Platelet factor-4) and SCYB4. The platelets, the most abundant source of CXCL4 in vivo, control profibrotic macrophage activation via CXCL4. (PMID: 36807143). Exogenous CXCL4 drove profibrotic activation of monocyte-derived dendritic cells in vitro (PMID: 33042127). Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation.
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文献和实验Chromosome Conformation Capture (3C) (alternate technique)
Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus (Dekker et al ., 2002). Cells are fixed with formaldehyde
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
较重要的是pH值和离子强度。PeproTech的细胞因子或重组蛋白在出厂前均经严格测试，说明上所标明的溶解液是能够将该细胞因子或重组蛋白完全溶解的液体。如果您所用的溶解液的pH值和离子强度与说明书中所标明的不符，很多时候会造成细胞因子或重组蛋白不能完全溶解或者根本无法溶解，这样所配得的细胞因子或重组蛋白必然活性不够或丧失。 有不少用户没注意说明书上的描述，而是根据习惯，直接用PBS或培养液(1640或DMEM等)等溶解细胞因子或蛋白的冻干粉。这样做可以吗？ 答：有时可以，要看具体
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