Raf1 (Phospho-Ser43) Rabbit mA

别名:C-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1;
计算分子量:73 kDa
配方:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:WB:1:500~1:1000
图片:

All lanes : Phospho-c Raf (S43) Rabbit mAb at 1/1k dilution
Lane 1 : MCF7 whole cell lysates
Lane 2 : Hela whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lane 4 : 293T whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 65-75 kDa
Exposure time: 10 seconds

,

All lanes : Phospho-c Raf (S43) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : Mouse Heart lysates
Lane 3 : Rat Brain lysates
Lysates/proteins at 20 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 65-75 kDa
Exposure time: 10 seconds

,

All lanes : Phospho-c Raf (S43) Rabbit mAb at 1/1k dilution
Lane 1 : MCF7 whole cell lysates
Lane 2 : MCF7 treated with 200nM PMA for 30min whole cell lysates
Lane 3 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 65-75 kDa
Exposure time: 7 seconds