- 询价
- Signalway Antibody
- 63752
- 2025年09月22日
- WB;IHC;IF;FC
- Mouse
- Human,Mouse,Monkey,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human HSPA9 (AA: 480-679) expressed in mammalian.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human,Mouse,Monkey,Rat
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB;IHC;IF;FC
- 靶点：
Uniprot：P38646
- 抗体英文名：
HSPA9 Mouse mAb
- 规格：
50ul/100ul
计算分子量:74KDa
配方:Purified antibody in PBS with 0.05% sodium azide
应用详情:WB:1/500 - 1/2000
IHC:1/200 - 1/1000
ICC:1/200 - 1/1000
FC:1/200 - 1/400
ELISA:1/10000
图片:
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
,
Western blot analysis using HSPA9 mouse mAb against A549 (1), PANC-1 (2), PC-12 (3), C6 (4), CSO-7 (5)and NIH3T3 (6) cell lysate.
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Immunofluorescence analysis of Hela cells using HSPA9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using HSPA9 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of Jurkat cells using HSPA9 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of HepG2 cells using HSPA9 mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissues using HSPA9 mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human rectal cancer tissues using HSPA9 mouse mAb with DAB staining.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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