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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of CD40 expressed in E. Coli.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Mouse
- 应用范围:
IF
- 靶点:
Uniprot:P25942
- 抗体英文名:
CD40 Mouse mAb
- 规格:
50ul/100ul
计算分子量:30.6kDa
配方:Ascitic fluid containing 0.03% sodium azide.
应用详情:ICC:1/200 - 1/1000
ELISA:1/10000
图片:

Confocal Immunofluorescence analysis of human peripheral blood lymphocyles (left) and mouse L1210 cells (right) using CD40 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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