- 询价
- Signalway Antibody
- 60290
- 2025年09月22日
- WB;ICC/IF;IHC
- Recombinant Rabbit
- Human;Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human Phospho-PKC alpha (T497)
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human;Mouse ,Rat
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB;ICC/IF;IHC
- 靶点：
Uniprot：P17252
- 抗体英文名：
PKC alpha (Phospho-Thr497) Antibody
- 规格：
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:
Western blot analysis of Phospho-PKC alpha (T497) expression in HeLa treated with Calyculin A cell lysate.
,
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
,
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
,
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
licanming 我的实验是用VEGF处理细胞后测定细胞PKC激活水平，拟采用Western blot的方法，用ECL法检测，这样分析PKC激活程度结果国际上认可吗? 我在Cell Signaling Technology上，查到 2261 Phospho-(Ser) PKC Substrate Antibody W IP E D 9371 Phospho-PKC (pan) (betaII Ser660) Antibody W IP
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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