- 询价
- Signalway Antibody
- 60290
- 2025年09月22日
- WB;ICC/IF;IHC
- Recombinant Rabbit
- Human;Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human Phospho-PKC alpha (T497)
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human;Mouse ,Rat
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB;ICC/IF;IHC
- 靶点：
Uniprot：P17252
- 抗体英文名：
PKC alpha (Phospho-Thr497) Antibody
- 规格：
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:
Western blot analysis of Phospho-PKC alpha (T497) expression in HeLa treated with Calyculin A cell lysate.
,
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
,
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
,
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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文献和实验licanming 我的实验是用VEGF处理细胞后测定细胞PKC激活水平，拟采用Western blot的方法，用ECL法检测，这样分析PKC激活程度结果国际上认可吗? 我在Cell Signaling Technology上，查到 2261 Phospho-(Ser) PKC Substrate Antibody W IP E D 9371 Phospho-PKC (pan) (betaII Ser660) Antibody W IP
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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