- 询价
- Signalway Antibody
- 30648
- 2025年09月23日
- WB,IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB,IF
- 靶点：
Uniprot：P62805
- 抗体英文名：
Acetyl-Histone H4-K16 pAb
- 规格：
50ul/100ul
计算分子量:13kDa
配方:Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
应用详情:WB 1:500 - 1:2000
IF 1:50 - 1:200
ChIP 1:50 - 1:200
图片:
Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37?? for 18 hours. Blue: DAPI for nuclear staining.
,
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37?? for 18 hours. Blue: DAPI for nuclear staining.
,
Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K16 (A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37?? for 18 hours. Blue: DAPI for nuclear staining.
,
Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H4-K16 Rabbit pAb (red, A5280) at dilution of 1:100. Blue: DAPI for nuclear staining.
,
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K16 antibody at 1:1000 dilution.
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文献和实验INTRODUCTION In cells and tissues, the histone proteins that constitute the nucleosomes can present multiple post-translational modifications, such as lysine acetylation, lysine and arginine methylation, serine phosphorylation
hyperphosphorylated H1 (pH1) from cells arrested in M phase by treatment with Colcemid (demecolcine; 0.06 µg/ml). The un‐ (Ac 0) and monoacetylated (Ac 1) isoforms of histone H4 are indicated. View Image
浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
[4] 。由于在信号转导和表观遗传研究中的突出作用，ChIP 在肿瘤 [5-7] 、神经科学 [8-10] 、植物发育 [11-13] 等领域中应用非常广泛，同时有关细胞凋亡 [14] 、雌激素信号转导 [15] 、胰岛素抵抗 [16] 、组织发育 [1] 的文献中也用到ChIP。 目前，最常见的有以下两种 ChIP 实验技术： nChIP：用来研究 DNA 及高结合力蛋白，采用微球菌核酸酶（micrococcal nuclease）消化染色质，然后进行片段富集及后续分析，适用于组蛋白及其异构体，例如 [17
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