- 询价
- Signalway Antibody
- C27080
- 2025年09月23日
- WB, IF, FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide (NPIQGLASKWDYEKNEWKKC) of human COX4(C-terminus).
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB, IF, FC
- 靶点：
Uniprot：Q96KJ9
- 抗体英文名：
COX4(isoform1) Conjugated Monoclonal Antibody
- 规格：
50ul/100ul
计算分子量:20
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
FC: 1:50-1:200
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文献和实验Western Blotting with Monoclonal Ant
the blocking buffer from the blot, add the antibody solution, and incubate with agitation for 30 minutes at 37˚C, one hour at room temperature, or overnight at 4˚C. Enzyme conjugated secondary antibody Note: The inclusion of sodium azide is to be avoided
Polyclonal and Monoclonal Antibodies
the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Immunization of Rabbits. Rabbits
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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