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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of threonine743 /668 (A-V-T(p)-P-E) derived from Human Amyloid β A4.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu Ms Rt
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P05067
- 抗体英文名:
Amyloid β A4 (Phospho-Thr743/668) Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:140
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验的。首先 APP 由β-分泌酶从 Aβ区的 N 端分泌产生可溶性 APP N 端片段β-APPs,和含有 Aβ膜相关性的 C 端片段(βCTF),然后经γ-分泌酶继续裂解,产生一系列长短不等的 Aβ(39~43 个氨基酸)和 AICD[4]。当 Aβ产生和清除之间的平衡就被打破,引起 Aβ在大脑皮质异常沉积,即导致神经突触功能损伤和神经元变性。 APP 的代谢途径 图片来源:Zhang and Song Alzheimer's Research & Therapy
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
Assays (See Note 9) 1. Pre-incubate anti-GSK3β (5 μg) monoclonal antibody withprotein G-Sepharose (400 μL of a 50% slurry) in kinasebuffer at 4°C for 1 h to conjugate the antibody to the proteinG-Sepharose beads. 2. Centrifuge the beads at 6,000× g
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
involves conventional 3C methodology: The chromatin is cross-linked, digested with restriction enzymes, and ligated under conditions that favor intramolecular ligation. Immediately after ligation, the chromatin is immunoprecipitated using an antibody
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