- 询价
- Signalway Antibody
- C11316
- 2025年09月23日
- WB, IF
- Rabbit
- Hu Ms
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of tyrosine 349 (H-Q-Y(p)-Y-N) derived from Human Shc1.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Hu Ms
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IF
- 靶点：
Uniprot：P29353
- 抗体英文名：
Shc1 (Phospho-Tyr349) Conjugated Antibody
- 规格：
50ul/100ul
计算分子量:46 52
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
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