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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from Human Keratin 8 (CK8).
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P05787
- 抗体英文名:
Keratin 8 (Phospho-Ser74) Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:55
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
-treatment conditions.Histochem . J. 18 :271. Rodriguez, J., and F. Deinhardt. 1960. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology 12 :316. Rosenberg, W.S., X.O. Breakefield, C. DeAntonio, and O
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