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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of serine 373 derived from Human Myc.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu Ms Rt
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P01106
- 抗体英文名:
Myc (Phospho-Ser373) Conjugated Antibody
- 规格:
50ul/100ul
计算分子量:60
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Twin-Site ELISAs for fos and myc Oncoproteins Using the AMPAK System
Twin-site ELISA is a simple technique for quantitation of specific proteins in cell or tissue extracts. The application of this method to fos and myc oncoproteins is described. There are two basic procedures:
-mediated reporter DNA liberation and Ru(bpy)3 2+ -conjugated silica nanoparticles (RuSi@Ru(bpy)3 2+ ) tag-based signal amplification.
0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated
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