- 询价
- Signalway Antibody
- C41553
- 2025年09月23日
- WB, IF
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from the Internal region of human IL-10.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IF
- 靶点：
Uniprot：P22301
- 抗体英文名：
IL-10 Polyclonal Conjugated Antibody
- 规格：
50ul/100ul
计算分子量:20
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
Polyclonal Antibody Production
to inactivate complement. Ring the inside walls of the tube with a glass pipet and place at 4 °C overnight to clot. Decant the clotted blood into a centrifuge tube and centrifuge at 4 °C 10,000g for 10
. at 37°C or O.N at 4°C. Separate the clot from the sides of the tube (ringing) using a pasteur pipette. Place clot at 4°C O.N. Spin at 10000 x g for 10 min. at 4°C to separate the serum. Serum can be stored at -20°C after adding Glycerol
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