FLCN Conjugated Antibody[C3648

Western Blot Analysis of Epitoped-tagged Proteins Using The Chemifluorescent Detection Method - for alkaline phosphatase conjugated antibodies

0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated

Nature重磅技术TransTAC选中的受体！TFR1这个铁转运蛋白要火了

明确。因此，还需要更深入地研究和探索。 TFR1的表达受多种刺激条件调控。在转录水平中，当细胞发生缺氧时，缺氧诱导因子（HIF）和其他转录因子如c-Myc、GATA1、Ets-1以及促红细胞生成素Stat5可以促进其转录。TFR1的转录后水平主要由IRP1和IRP2调控，它们与TFR1 mRNA中的IRE结合来影响基因表达 [13-15]。 在翻译后水平，CD133（PROM1）是TFR1转运铁元素过程中的负调节因子，同时EGF受体、c-Abl分子和MARCH8分子可能也参与其中。如下图所示，一项