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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic methylated peptide corresponding to residues surrounding Arg26 of human histone H3
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Hu Ms Rt Other (Wide Rtange)
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF
- 靶点:
Uniprot:P68431
- 抗体英文名:
Histone H3R26me2sConjugated Antibody
- 规格:
50ul/100ul
计算分子量:15
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验9.19 Nature:揭示组蛋白标记 H3K36me2 招募 DNMT3A 并影响基因间 DNA 甲基化
中心、洛克菲勒大学和加拿大麦吉尔大学等研究机构的研究人员报道 NSD1 介导的 H3K36me2 是在基因间区域招募 DNMT3A 和维持 DNA 甲基化所必需的。这项新的研究揭示了一种跨染色质调节途径,这种调节突进将异常的基因间 CpG 甲基化与人类肿瘤生长和过度发育相关联在一起。原文检索:The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape ② Nature 突破
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
control) relative to the level of the signal of interest (ChIP sample). This hampers distinguishing a ''true signal'' from the background signal. Therefore, we recommend setting up ChIP using an antibody against an invariant domain of histone H3 [19,20
at lysine 4 of H3 (H3K4Me2; Upstate Ltd.). Radioactive PCR was performed on bound (B) and unbound (U) fractions with primers that amplified from a unique sequence at an imprinting-control center located in a gene called Kvlqt1. The PCR products
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