Gelsolin Conjugated Antibody[C

人凝溶胶蛋白(Gelsolin)ELISA试剂盒 说明书

  上海西唐生物科技有限公司   021-55229872,  65333639   www.westang.com 人 凝溶胶蛋白 ( Gelsolin )ELISA 试剂盒   原理 本实验采用双抗体夹心  ABC-ELISA 法。用抗人   Gelsolin   单抗包被于酶标板上，标准品和样品中的   Gelsolin与单抗结合，加入生物素化的抗人 Gelsolin ，形成免疫复合物连接在板上，辣根

Western Blot Analysis of Epitoped-tagged Proteins Using The Chemifluorescent Detection Method - for alkaline phosphatase conjugated antibodies

0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated