IL1 beta Conjugated Antibody[C

Western Blot Analysis of Epitoped-tagged Proteins Using The Chemifluorescent Detection Method - for alkaline phosphatase conjugated antibodies

0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated

Large-scale immunofluorescence

overnight7 at 4o C with very gentle shaking. Wash cells once with PBS, once with PBS plus 0.1% NP40, and then again with PBS. Resuspend cells in 30 ul of PBS plus 0.1% BSA containing the secondary antibody8 , and incubate for 4 hours at room

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated