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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
recombinant protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Hu, Ms, Rt
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB, IF, FC
- 靶点:
Uniprot:Q9Y478
- 抗体英文名:
AMPK beta 1 Conjugated Antibody
- 规格:
50ul/100ul
5''-AMP-activated protein kinase subunit beta-1 antibody
5'-AMP-activated protein kinase beta-1 subunit antibody
AAKB1_HUMAN antibody
AMP-activated protein kinase beta subunit antibody
AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1 antibody
AMP-activated, noncatalytic, beta-1 antibody
AMPK antibody
AMPK beta 1 chain antibody
AMPK subunit beta-1 antibody
AMPK-BETA-1 antibody
AMPKb antibody
AU021155 antibody
E430008F22 antibody
HAMPKb antibody
MGC17785 antibody
PRKAB1 antibody
Protein kinase AMP activated non catalytic subunit beta 1 antibody
protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody
protein kinase, AMP-activated, noncatalytic, beta-1 antibody
计算分子量:38 kDa
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
FC: 1:50-1:200
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文献和实验Large-scale immunofluorescence
overnight7 at 4o C with very gentle shaking. Wash cells once with PBS, once with PBS plus 0.1% NP40, and then again with PBS. Resuspend cells in 30 ul of PBS plus 0.1% BSA containing the secondary antibody8 , and incubate for 4 hours at room
2 n-propyl gallate and 0.25 ug/ml Hoechst Primary antibodies rabbit anti-beta-gal antibody (Cappell Laboratories #0631-0002, at 1:200 dilution) rat monoclonal anti-yeast tubulin (YOL1/34; Sera-lab, at 1:100 dilution
Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
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