WNT3A/WNT3 Conjugated Antibody

v468 Chapter 2 Wnt活性蛋白分离及应用

Systemor equivalent). 4. Nitrocellulose or polyvinyl difluoride (PVDF) membrane. 5. Wnt3A antibody (available from R&D Systems, Minneapolis,MN). 6. Appropriate conjugated secondary antibody for detection. 2.4.2. b-Catenin Stabilization

Western Blot Analysis of Epitoped-tagged Proteins Using The Chemifluorescent Detection Method - for alkaline phosphatase conjugated antibodies

0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated

v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

phosphate-buffered saline (PBS) containing 0.1% (w/v)bovine serum albumin (BSA) at a concentration of 50 μg/mLand stored in aliquots at –20°C (see Note 2 ). 3. Mouse L cells that express and secrete Wnt3a (ATCC, Manassas,VA; #CRL-2647) and the control L