- 询价
- Signalway Antibody
- C48066
- 2025年09月23日
- WB, IF
- Mouse
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
protein
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Mouse
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB, IF
- 抗体英文名：
kinase D-interacting substrate 220 Conjugated Antibody
- 规格：
50ul/100ul
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Phage‐Based Expression Cloning to Identify Interacting Proteins
F‐like properties. Cell 70:351‐364. Lester, L.B., Coghlan, V.M., Nauert, B., and Scott, J.D. 1996. Cloning and characterization of a novel A‐kinase anchoring protein: AKAP220, association
Immunoprecipitation and Immune Complex kinase assay
per 10 microliters KB. When using an exogenous substrate such as enolase, add the substrate to the ATP mix. 3) Add 10 microliters ATP mix to each IP, pipetting to mix, then incubate at 30°C for 10 min. 4) Stop kinase assay by adding 1 ml of TN + 0.01
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Doppler, H., Storz, P., LI, J., Comb, M. J., and Toker, A. 2005. A phosphorylation state‐specific antibody recognizes Hsp27, a novel substrate of protein kinase D. J. Biol. Chem. 280:15013‐15019
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