- 询价
- Signalway Antibody
- C29229
- 2025年09月23日
- WB, IF
- Rabbit
- Hu Ms Rt
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human PLCG2
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Hu Ms Rt
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB, IF
- 靶点：
Uniprot：P16885
- 抗体英文名：
PLC gamma 2 Conjugated Antibody
- 规格：
50ul/100ul
计算分子量:150
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
Intracellular Cytokine Staining Protocol
. Some protocols include a step of airfuging the antibody prior to multi-color flow analysis. For optimal performance of fluorochrome conjugated antibodies, wrap the vials with aluminum foil and store at 4°C in the dark. Do not freeze. When staining
the appropriate quantity of Sepharose CL-6B beads (30 µl/sample) with hypotonic buffer and mix with FLAG-M2 agarose beads (10 µl/sample), or beads conjugated to another antibody to the chosen epitope tag (~1 µg of antibody per 1 mg of protein lysate).
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