- 询价
- Signalway Antibody
- C92459PE
- 2025年09月22日
- FC;IF
- Rabbit
- Hu Ms Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Hu Ms Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
FC;IF
- 靶点：
Uniprot：P34995
- 抗体英文名：
Prostaglandin E Receptor EP1 Polyclonal Antibody PE Conjugated
- 规格：
50ul/100ul
计算分子量:42
配方:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
应用详情:Flow-Cyt=1:50-200 IF=1:50-200
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文献和实验Phycoerythrin conjugation protocol
Centricon 30 and Centriprep 30 centrifugal concentrators (Amicon) * Room temperature is about 22 °C Method 1. Preparation of PE 2. SPDP modification of PE 3. SMCC modification of antibody 4. DTT
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Immunofluorescent Staining of Mouse and Rat Leukocytes
secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet
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