- 询价
- Signalway Antibody
- 13822
- 2025年09月22日
- IHC-p,IF/ICC,ELISA
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against synthesized peptide derived from human Histone H3.3 around the phosphorylation site of Ser31. AA range:16-65
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
IHC-p,IF/ICC,ELISA
- 靶点：
Uniprot：P84243
- 抗体英文名：
Histone H3.3 (phospho-Ser31) Polyclonal Antibody
- 规格：
50ul/100ul
计算分子量:15kd
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/20000. Not yet tested in other applications.
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
Growth factor stimulation results in phosphorylation of histone H3 at ser 10 and this correlated with expression of immediate early genes suggesting that this phosphorylation is associated with transcriptional activation. Although Western
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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