- 询价
- Signalway Antibody
- 13435
- 2025年09月23日
- WB;ICC/IF;IHC
- Recombinant Rabbit
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic phospho-peptide corresponding to residues surrounding Ser133 of human Creb.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 适应物种：
Human;Mouse
- 保质期：
12 months
- 抗原来源：
Recombinant Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Recombinant Rabbit
- 应用范围：
WB;ICC/IF;IHC
- 靶点：
Uniprot：P16220
- 抗体英文名：
Creb (Phospho-S133) Rabbit mAb
- 规格：
50ul/100ul
cAMP response element binding protein 1 antibody
cAMP response element binding protein antibody
cAMP responsive element binding protein 1 antibody
cAMP-responsive element-binding protein 1 antibody
CREB antibody
CREB-1 antibody
CREB1 antibody
CREB1_HUMAN antibody
Cyclic AMP-responsive element-binding protein 1 antibody
MGC9284 antibody
OTTHUMP00000163864 antibody
OTTHUMP00000163865 antibody
OTTHUMP00000206660 antibody
OTTHUMP00000206662 antibody
OTTHUMP00000206667 antibody
Transactivator protein antibody
计算分子量:35 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:
Western blot analysis of Phospho-Creb (S133) on human colon tissue lysates using anti-Phospho-Creb (S133) antibody at 1/500 dilution.
,
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
,
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Creb (S133) antibody. Counter stained with hematoxylin.
,
ICC staining Phospho-Creb (S133) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
ICC staining Phospho-Creb (S133) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,
Flow cytometric analysis of HUVEC cells with Phospho-Creb (S133) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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文献和实验which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
-Rabbit HRP. Wash filter 3 times 7 minutes with 0.5% BSA in TBST. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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