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文献和实验The Cell Environment (including types of culture medium)
free (Prod. No. W3500, water, tissue culture grade). In most cases water prepared by reverse osmosis and resin cartridge purification with a final resistance of 16-18Mx is suitable. Once prepared the media should be filter sterilized before use
The Cell Environment (including types of culture medium)
culture grade). In most cases water prepared by reverse osmosis and resin cartridge purification with a final resistance of 16-18Mx is suitable. Once prepared the media should be filter sterilized before use. Obviously purchasing 1x liquid media direct
Differentiate ES cells into glial cells and neurons
together by gentle handling. Then directly plate the cells 1:3 into bacterial grade Petri dishes in LIF free medium containing 1 microM all-trans retinoic acid (RA).Day 3 Collect cell aggregates and replate in tissue culture dishes (appr. 25 cell aggregates per 6 cm
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