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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic phospho-peptide corresponding to residues surrounding Ser641 of human Glycogen synthase 1.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Human;Mouse
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB;ICC/IF;IHC
- 靶点:
Uniprot:P13807
- 抗体英文名:
Glycogen synthase 1(Phospho-Ser641) Rabbit mAb
- 规格:
50ul/100ul
Glycogen synthase 1 (muscle) antibody
Glycogen synthase 1 antibody
GSY antibody
GYS antibody
Gys1 antibody
GYS1_HUMAN antibody
muscle antibody
计算分子量:84 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
图片:

Western blot analysis of Phospho-Glycogen synthase 1(S641) on different lysates using anti-Phospho-Glycogen synthase 1(S641) antibody at 1/1,000 dilution. Positive control:
Lane 1: Mouse liver lysate, untreated
Lane 3: Mouse liver lysate, treated with AP
,

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Phospho-Glycogen synthase 1(S641) antibody. Counter stained with hematoxylin.
,

ICC staining Phospho-Glycogen synthase 1(S641) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

ICC staining Phospho-Glycogen synthase 1(S641) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
solution contains 100 mMMgCl 2 , 1 mM un-labelled ATP, and approximately 30 MBq/mL [g- 32 P]ATP. 5. Phospho-glycogen synthase 2 (pGS2) peptide is available fromUpstate (#12-241) and the pGS2 10× stock solution contains200 μM pGS2 in kinase buffer
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