- 询价
- Signalway Antibody
- 12243
- 2025年09月22日
- WB IHC ELISA
- Rabbit
- Human;Mouse;Rat;Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthesized peptide derived from human hnRNP K around the phosphorylation site of S284.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat;Monkey
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC ELISA
- 靶点：
Uniprot：P61978
- 抗体英文名：
hnRNP K (Phospho-Ser284) Polyclonal Antibody
- 规格：
50ul/100ul
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:Western blotting: 1/500 - 1/2000
Immunohistochemistry: 1/100 - 1/300
ELISA: 1/20000
Not yet tested in other applications
图片:
Western Blot analysis of 3T3 cells using Phospho-hnRNP K (S284) Polyclonal Antibody
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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
(either undiluted or 1:1 with PBT) or diluted polyclonal antiserum and incubate at 4 C overnight 8. Rinse with PBT 3 times, then wash 4 times for 15 minutes each 9. Add secondary antibody (usually 1:200 in PBT) and incubate 2 hours at room temperature
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
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