- 询价
- Signalway Antibody
- 12133
- 2025年09月22日
- WB
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of threonine 100 (S-G-T(p)-Q-I) derived from Human CREB.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：P16220
- 抗体英文名：
CREB (Phospho-Thr100) Antibody
- 规格：
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Western blotting: 1:500~1:3000
图片:
Western blot analysis of extracts from HUVEC cells, treated with etoposide (25uM, 24hours), using CREB (Phospho-Thr100) antibody #12133. The lane on the right is treated with the synthesized peptide.
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文献和实验1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. Aspirate media. Rinse cells with PBS, aspirate. Lyse cells in 200 uL of SDS sample buffer (100 oC
secondary antibody review -- data from 99 publications
IgG Alexa Fluor 488 1:250 Molecular Probes 10 goat Cy3 immunocytochemistry detect NMDA receptors in HEK293 cells Jackson ImmunoResearch Laboratories 10 FITC immunocytochemistry 1:100 detect phospho-CREB in human colonic
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