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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized Acetyl peptide derived from human PARP-1. at AA range: K521
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
Uniprot:O60674
- 抗体英文名:
PARP-1 (Acetyl-K521) Polyclonal Antibody
- 规格:
50ul/100ul
计算分子量:113 kDa
配方:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
应用详情:Western blotting: 1£º500-1£º2000
图片:
Western Blot analysis of 1,mouse-heart 3,mouse-brain cells using primary antibody diluted at 1:1000(˚C overnight). Secondary antibody£oGoat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25??C, 1 hour)
,

Western Blot analysis of 1,293t 2,mouse-brain cells using primary antibody diluted at 1:1000(˚C overnight). Secondary antibody£oGoat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25??C, 1 hour)
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文献和实验of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) could be detected in the bound fraction after precipitation with conventional mono- and polyclonal antibodies (Figure 1) (Rothbauer et al., 2008). The lack of unspecific binding or antibody fragments is one major advantage of the GFP-Trap, because unspecific protein
Introduction of human 14-3-3 proteins
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
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