DGAT1 Antibody[37532]

别名:ARAT; DGAT; ARGP1
配方:0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
应用详情:WB1:500-2000
IHC 1:100-500
图片:

Sample: Lane 1: Mouse Adipose tissue lysates Lane 2: Mouse Adrenal gland tissue lysates Lane 3: Rat Adipose tissue lysates Lane 4: Rat Stomach tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Human HL60 cell lysates Lane 7: Human HepG2 cell lysates Primary: Anti-DGAT1 at 1/300 dilution Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 60 kD

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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGAT1) Polyclonal Antibody, at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.