- 询价
- Signalway Antibody
- 42283
- 2025年09月22日
- WB
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Histone H2B type 1-C/E/F/G/I protein
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：P62807
- 抗体英文名：
Histone H2B type 1-C/E/F/G/I Polyclonal Antibody
- 规格：
50ul/100ul
计算分子量:14kd
配方:Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
应用详情:Western blotting: 1:500 - 1:1000
图片:
All lanes : Histone H2B type 1-C/E/F/G/I antibody at at 2ug/mlLane 1 : EC109 whole cell lysateLane 2 : 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 14 kDa Observed band size: 70kDa
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文献和实验Polyclonal and Monoclonal Antibodies
1) After collecting, blood should be allowed to clot for 60 min . at 37 C or O.N at 4 C. 2) Separate the clot from the sides of the tube (ringing) using a pasteur pipette. Place clot at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate
:3 ratio (e.g. 1µl biotin-tagged RNA (for final 70-100nM concentration) to 3µl lipofectamine). However, the RNAs are first heated to 70°C for 10 minutes to allow the relaxation of any untoward secondary structures. The RNAs and lipofectamine are then mixed
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
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