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广州威佳科技有限公司
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滤膜孔径：0.22μm
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文献和实验Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
4. Wash cells by resuspending in 25 mls of ICE COLD 10mM NaCl (0.1ml 5M into 50ml final) per 250 ml bottle, pellet at 3800 rpm for 10minutes at 4°C. 5. Decant supernatant, (freezing the pellet at minus 80°C overnight at this step increases cell
细菌RNA的提取--ISOLATION OF RNA FROM BACTEROIDS
. at -80℃.ISOLATION OF RNA FROM FREE-LIVING RHIZOBIACells from 100 ml culture in early log phase GYPC were spun and resuspended in 1 ml sterile 25% sucrose. This solution was transferred to a sterile centrifuge tube and 6.5 ml M-STET, 0.5 ml lysozyme (10 mg
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