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- FP050D0QF1QUFC01
- 2025年09月08日
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- 文献和实验
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- 库存：
9999
- 供应商：
广州威佳科技有限公司
- 规格：
100片/盒
孔径：50mm
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文献和实验. Sponge - filter paper - gel - membrane - filter paper - sponge 3. Transfer for 1 hr at 1 amp at 4°C on a stir plate. Bigger proteins might take longer to transfer. For the Mini-Transblot, it's 100 V for 1 hr with the cold pack and prechilled buffer
BAC DNA Purification For Injection
Equilibrate tip-500 column with 10 ml QBT buffer (750 mM NaCl, 50mM MOPS pH 7.0, 15% isopropanol, 0.15% Triton X-100) Apply filtered DNA solution to the column Wash column twice with 30 ml QC buffer (1.0 M NaCl, 50 mM MOPS pH 7.0, 15% isopropanol
Preparation of Poly A+ RNA and Northern Analysis
. Holding the filter by two sides, carefully set down exactly on the gel (canÕt reposition). Carefully roll the capless conical over the filter to ensure removal of any air bubbles. Add the remaining gel-size piece of 3MM paper (wet with 10 x SSC) and roll
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