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- 2025年09月08日
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- 文献和实验
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- 库存：
9999
- 供应商：
广州威佳科技有限公司
- 规格：
100片/盒
孔径：50mm
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文献和实验. Sponge - filter paper - gel - membrane - filter paper - sponge 3. Transfer for 1 hr at 1 amp at 4°C on a stir plate. Bigger proteins might take longer to transfer. For the Mini-Transblot, it's 100 V for 1 hr with the cold pack and prechilled buffer
BAC DNA Purification For Injection
buffer (200 mM NaOH, 1% SDS); invert slowly 4-6 times; incubate 5 min at RT Add 50 ml pre-chilled P3 buffer; invert slowly 4-6 times; incubate on ice 30 min Spin 60 min at 15000xg at 4°C; filter supernatant through Whatman filter paper
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
(Vector Laboratories PK 6100) (optional, see Step 30) Wash solution 1 Wash solution 2 Equipment Aluminum foil Diamond pen Eye protection (see Step 21) Filter paper (e.g., Whatman 3MM) Humid chamber for slide
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