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文献和实验Expression Protocol in M9 Minimal Media via T7 Promoter
Medium Eagle Vitamin Solution (Gibco) 2.5 mL filter sterilized NH4 Cl (0.2 g/mL) or 0.5g dry 10 mL 20% d-glucose or 2g dry glass distilled & autoclaved H2 O to final volume of 500mL pH solution to 7.3 and filter sterilize (0.2u
Expression Protocol in M9 Minimal Media via T7 Promoter
The following protocol has been used successfully to 15N or 13C/15N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 minimal media begins with preparing a 5x stock solution of M9 salts. Generally, M9 salts contain
RNA purification using TRizol--TRIZOL法提取RNA
microcentrifuge tube and pestle for homogenization, start with 500 μl of TRIzol or TRI reagent, then add remaining 500 μl.b.Homogenize the samples using a sterile or RNAse free plastic, glass pestles or power homogenizer and tubes.Modification for monolayersa.Lyse
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