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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. 36. Resuspend the cells in OP9 medium, seed 5 x 105 cells per 10-cm dish containing ~80% confluent OP9 cells in 10 mL of OP9 medium, and add 5 ng/mL of recombinant human Flt-3L. Return the dish to the incubator. The number of 10-cm dishes
Method for Single-Cell Electroporation
for other preparations. Equipment Microscope: dissecting scope, or upright with long working distance, low power (~20X) objective Voltage stimulator: Grass SD
Steady State ATPase Assays Coupled Enzyme System
340 at 10 second intervals for 300 seconds. A rate factor of 6.220 x 106 cm2 at 340 nm = molar extinction coefficient of NADH can be entered into the data collection program. 4. Wash and invert 4 cuvettes to dry
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