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- 详细信息
- 文献和实验
- 技术资料
- 库存:
9999
- 供应商:
广州威佳科技有限公司
- 规格:
25片/盒
孔径:293mm
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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
Construction and Characterization of Adenovirus Vectors
. Add 3 mL to each 35-mm dish of 293 cells. This must be performed quickly to avoid solidification of the agarose, but gently to avoid disturbing the monolayer. 25. Allow the overlay to solidify (~15 min at 22°C).
µCi/µl, specific activity 3000 Ci/mmol) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)dNTPs (stock solutions at 25 mM for each dNTP)DNA loading buffer (6X)30% (v/v) glycerol0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FFStore
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