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文献和实验Steady State ATPase Assays Coupled Enzyme System
. 5. Make Tris-MgCl2 -DTT mix for all assays. Tris-MgCl2 -DTT mix = (n = # of assays + 1) n x 20 µL 0.5 M Tris-OAc, pH 7.5 20 µL 10 mM MgCl2 20 µL 10 mM DTT 70 µL DDW
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
!!). Place this carefully into the 0.5 ml pcr tube with the pcr/pk buffer. The amount of pcr/pk buffer depends on the sample. In general it's 15 ul for anything less than 1 mm, and proportionally more if larger. Go onto next area. Remember to use
Bromodeoxyuridine Immunohistochemistry
between the following steps, and use apx. 20 ml for each reagent (step 3-7). 6. 30, 50, 70, 95, 100 x 2, RT, 30 min. each , with slight agitation. This step functions to dehydrate the tissues through an increasing ethanol series: Samples in 70, 95, or 100
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