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文献和实验Apoptosis Measurement by Annexin V Staining
is associated with the condensed chromatin in apoptotic cells as a measure of apoptosis. It may appear as early as 3 h post the apoptosis inducing event and precedes the ability to detect actual DNA fragmentation. The annexin V assay has been widely accepted
Detection of Apoptosis in Ovarian Cells In Vitro and In Vivo Using the Annexin V-Affinity Assay
can be detected in vitro and in vivo with fluorochrome- or biotin-labeled annexin V, a protein that binds to negatively charged phospholipids in the presence of calcium ions ( 6 , 7 ). In cells that are cultured in suspension, detection of apoptosis on the basis
. During apoptosis, PS is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2 -dependent affinity for PS and therefore can be used as a probe for detecting apoptosis. 实验步骤 Example
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