- ￥462 - 978
- MCE
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- HY-115374
- 2025年09月06日
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- 技术资料
- 保存条件：
见包装
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- 库存：
10
- 供应商：
广州市左克生物
- CAS号：
1043884-49-6
- 规格：
100mg/250mg
| 规格： | 100mg | 产品价格： | ￥462.0 |
|---|---|---|---|
| 规格： | 250mg | 产品价格： | ￥978.0 |
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文献和实验Crystallization of Kinesin Family Motor Proteins
MgCl2, 2 mM DTT was pre-incubated with 4 mM AMP*PNP or ATP for 2 hoursCrystals grew in 11% PEG 8000, 0.8 M NaCl, 50 mM Na2H2PO4, pH 6.8, 7 mM DTTYun et al., 2003Rat Kinesin-1 rK354Hanging drop Room temperature9-14 mg/ml protein 20 mM PIPES pH 7.5, 50 mM
Monoclonal Antibody Production Protocol
the PEG. Do not disturb the cells. Spin at 1000 rpm for 5 min. Aspirate medium, resuspend cells by tapping. Slowly add 5 ml of 37°C IMDM-m. Bring to 20 ml and add to the flask in the incubator. (If using feeder cells, add them at this point; 106 cells
Replication timing by density transfer
for ~1 min. You can empirically determine how long it takes to bring your desired volume of medium from 37o to 23o C. 6. Collecting samples: Freeze 8 ml of 0.1% Na-azide, 0.2 M EDTA in "50 ml" Oakridge-type plastic, screw-capped centrifuge tubes
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