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- 2025年09月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见包装
- 保质期：
其他规格、详情咨询客服
- 库存：
10
- 供应商：
广州市左克生物
- CAS号：
1448821-89-3
- 规格：
1mg
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
siliconized slide place two drops of 45% acetic acid and a clean siliconized 18 mm square coverslip. On the coverslip place a small drop (2-3 mm) of fixative( 1/2/3 mixture of lactic acid/water/glacial acetic acid) 3) In the first drop of 45% acetic
Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
for 14 days at 37°C. 11. Between 3 and 7 days and 10 and 14 days incubation, subculture 0.1 ml of test broth ontoan agar plate and incubate plate anaerobically as above. 12. Observe agar plates after 14 days incubation at x300 magnification using
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