5,6-Diamino-1,10-phenanthrolin

Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper

The existence of cell-type specific promoter and enhancer elements has been known for several years. However, the mechanisms responsible for the remarkable specificity of such elements, in comparison to the ubiquitously active promoters

SINGLE STEP PROKARYOTIC RNA ISOLATION

2-mercaptoethanol        10 mM           4.4 mM 5% SDS - 1 gram per 20ml 2.3 mg/ml Protease K - 46 mg in 20ml ddH2O 0.1M EDTA - 4ml 0.5M EDTA + 16ml ddH2O 20mM 1,10-phenanthroline - 79 mg in 20ml ddH2O(boil to dissolve) 2 mg/ml Heparin - 40 mg in 20ml ddH

Footprinting DNAProtein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-PhenanthrolineCopper

, with the subsequent exposure of the resolved DNA–protein complex(es) to the chemical nuclease 1,10-phenanthroline–copper ion (OP–Cu) while they are still embedded in the polyacrylamide matrix (in-gel assay ).