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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
其他规格、详情咨询客服
- 库存:
10
- 供应商:
广州市左克生物
- CAS号:
0
- 规格:
5mg/10mg/25mg
| 规格: | 5mg | 产品价格: | ¥372.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥595.0 |
| 规格: | 25mg | 产品价格: | ¥1192.0 |
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文献和实验Coupling Peptides To Resin For Affinity Purification
5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate
Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
Revised amino-allyl labeling protocol
, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution. 3. Add 450 m L water to each reaction. C. Cleanup Add 500 m L neutralized, diluted reaction
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