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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
其他规格、详情咨询客服
- 库存:
10
- 供应商:
广州市左克生物
- CAS号:
1246303-22-9
- 规格:
500ug
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文献和实验The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
. Incubate the samples for 30 min at 37°C. 22. Extract once with an equal volume of Phenol:Chloroform. Mix well. 23. Centrifuge at 16,000 X g at room temperature for 10 min. 24. Collect the upper, aqueous phase. 25. Add 7.5 M Ammonium Acetate, pH
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