- 询价
- Signalway Antibody
- 11541
- 2025年09月04日
- WB
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of threonine 395 (L-L-T(p)-P-P)derived from Human Cyclin E1
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
Uniprot：P24864
- 抗体英文名：
Cyclin E1(phospho-Thr395) Antibody
- 规格：
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Predicted MW: 48kd
Western blotting: 1:1000
图片:
Western blot analysis of extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1(phospho-Thr395) Antibody #11541.
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文献和实验microRNA-195通过直接影响靶基因Cyclin D1和Cyclin E1的表达 抑制了人神经胶质瘤细胞的增殖
膜母细胞瘤（pRb）和增殖细胞核抗原（PCNA）的形成。相反，抑制了miR-195的作用后，神经胶质瘤细胞的增殖速度加快，S期细胞的比例升高，G1/G0期的细胞数减少，提高细胞的非依赖型锚定生长能力，促进细胞中pRb和PCNA的磷酸化。此外，我们还发现miR-195通过直接作用于Cyclin D1和Cyclin E1的mRNA 3’UTR区下调Cyclin D1’和Cyclin E1的表达，抑制了神经胶质瘤细胞的增殖。综上所述，该实验结果表明miR-195在抑制神经胶质瘤细胞增殖的过程中发挥了重要的作用，并阐明
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis
and members of the Bcl-2 protein family. In this chapter, we describe methods for measuring changes in Cdk2 activity during apoptosis. In addition, we also show the details of the generation of an antibody able to immunoprecipitate the cyclin O complexes
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