- 询价
- Signalway Antibody
- 11224
- 2025年09月04日
- WB IHC
- Rabbit
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of serine 25 (I-L-S(p)-P-R) derived from Human Stathmin 1.
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC
- 靶点：
Uniprot：P16949
- 抗体英文名：
Stathmin1(Phospho-Ser25) Antibody
- 规格：
50ul/100ul
配方:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
图片:
Western blot analysis of extracts from Jurkat cells untreated or treated with PMA using Stathmin 1(Phospho-Ser25) Antibody #11224.
,
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Stathmin 1(Phospho-Ser25) Antibody #11224(left) or the same antibody preincubated with blocking peptide(right).
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
双特异抗体(Bispecific antibody)的简介和制作方法
linker。目前用于该设计方法的linker 有：25 氨基酸残基的205c linker，以2C11CH1片段为主的23 氨基酸残基linker，24氨基酸残基的CBH1 linker，114氨基酸残基的ETA Ⅱ区段linker，（Gly4Ser）3及Ser2 （Gly4Ser）3linker等，均获得了有功能的双特异抗体分子。Coloma等将一种scFv直接融合表达于另一种scFvC 端或铰链区之后也获得了有功能的bis2Fv。Helfrich 等还构建了专门用于表达bisFvs的载体，可直接
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