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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant human FOS protein fragments expressed in E.coli.
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Hu Rt Mk
- 保质期:
12 months
- 抗原来源:
Mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Mouse
- 应用范围:
WB
- 靶点:
Uniprot:P01100
- 抗体英文名:
c-Fos Monoclonal Antibody
- 规格:
50ul/100ul
配方:Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
应用详情:
Western blotting: 1:500
图片:

Western blot detection of c-Fos antibody in HCT116,COS7 and C6 cell lysates using c-Fos antibody (1??00 diluted).Predicted band size:62KDa.Observed band size:62KDa.
,

Western blot detection of c-Fos antibody in Hela(TSA treated ) and Hela cell lysates using c-Fos antibody (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.
,

Western blot analysis of extracts from Hela cells,untreated,50 μM TSA-treated or 50 μM TPA-treated for 24h,using C-FOS antibody (1:500 diluted)(upper) or GAPDH antibody (1:10000 diluted) (lower).
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody-Based Immunoassays
An immunoassay may be defined as an assay that employs an immunological reagent, usually an antibody, to confer specificity for the ligand being measured. As a corollary to this, the discovery, and subsequent development, of monoclonal
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