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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant fragment , corresponding to amino acids 131aa-389aa of human PDHA1 (C-terminus).
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal
- 适应物种:
Hu D Rt Ms P
- 保质期:
12 months
- 抗原来源:
Mouse
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Mouse
- 应用范围:
WB ICC
- 靶点:
Uniprot:P08559
- 抗体英文名:
Pyruvate Dehydrogenase(C-term) Monoclonal Antibody
- 规格:
50ul/100ul
配方:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
应用详情:
Predicted MW: 50kd
Western blotting: 1:1000
图片:

1:3000 dilution of this antibody detected PDHA1 on 40ug of cell lysates
,

HeLa cells using anti- PDHA1 (C-terminus) antibody diluted 1:150
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
关于内参,已经有很多的帖子讨论,但是在我们的动物或细胞的模型中,总是有各种干扰的过程常常影响内参的表达,比如GAPDH就参与糖代谢,变性性疾病,凋亡等多种过程,如果我们的试验中存在这种过程干扰内参的表达,应该如何选择呢?Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody Glyseraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme
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